Conserved OprF as a Selective Immunogen Against Pseudomonas aeruginosa

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Conserved OprF as a Selective Immunogen against Pseudomonas aeruginosa

Background & Objectives Due to the importance of Pseudomonas aeruginosa in severe inpatient infections and high mortality, the need for an efficient vaccine against these bacteria is increasing. In this regard, the general outer membrane porin of the most problematic microorganism P. aeruginosa, outer membrane protein F (OprF), is a good vaccine candidate. Methods The databank of NCBI was use...

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OprF and OprL Conjugate as Vaccine Candidates against Pseudomonas aeruginosa; an in Silico Study

Introduction: Vaccine studies against Pseudomonas aeruginosa have often focused on outer membrane proteins (OPRs) due to their potent stimulation of the immune response. Using major outer membrane proteins of cell walls (mOMPs) of P. aeruginosa and other Gram-negative bacteria actively stimulate the immune system without any toxic side effects. Moreover, these antigens show immunological cross-...

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Linker-insertion mutagenesis of Pseudomonas aeruginosa outer membrane protein OprF.

The oprF gene, expressing Pseudomonas aeruginosa major outer membrane protein OprF, was subjected to semi-random linker mutagenesis by insertion of a 1.3 kb Hincll kanamycin-resistance fragment from plasmid pUC4KAPA into multiple blunt-ended restriction sites in the oprF gene. The kanamycin-resistance gene was then removed by Pstl digestion, which left a 12 nucleotide pair linker residue. Nine ...

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Manuka honey is bactericidal against Pseudomonas aeruginosa and results in differential expression of oprF and algD.

The presence of Pseudomonas aeruginosa in cutaneous wounds is of clinical significance and can lead to persistent infections. Manuka honey has gained ground in clinical settings due to its effective therapeutic action and broad spectrum of antibacterial activity. In this study, the effect of manuka honey on P. aeruginosa was investigated using MIC, MBC, growth kinetics, confocal microscopy, ato...

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ژورنال

عنوان ژورنال: Iranian Journal of Pathology

سال: 2017

ISSN: 2345-3656

DOI: 10.30699/ijp.2017.24875